Recombinant humaan CHCHD7 eiwit

CHCHD7 is a newly identified member of a multifamily of proteins containing a conserved (coiled coil 1) - (helix 1) - (coiled coil 2) - (helix 2) domain. Its biological function is unknown and the gene has hitherto not been associated with neoplasia. CHCHD7 and PLAG1 are located head-to-head about 500 bp apart in 8q12. Molecular analyses of 27 tumors revealed CHCHD7-PLAG1 fusions in three tumors, two of which had t (6;8) and t (8;15) translocations as the sole anomalies and one a normal karyotype. Recombinant human CHCHD7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Coiled-coil-helix-coiled-coil-helix domain-containing protein 7, COX23

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

13.9 kDa (120aa) confirmed by MALDI-TOF

CHCHD7, Coiled-coil-helix-coiled-coil-helix domain-containing protein 7, COX23, ATGP2814-10 µg, ATGP2814-20 µg, ATGP2814-50 µg, ATGP2814-100 µg, ATGP2814-250 µg, ATGP2814-500 µg, ATGP2814-1 mg, ATGP2814-10, ATGP2814-20, ATGP2814-50, ATGP2814-100, ATGP2814-250, ATGP2814-500, ATGP2814-1

Asp J. et al. (2006) Genes Chromosomes Cancer. 45:820-828; ; Banci L. et al. (2012) J Struct Biol. 180:190-200.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Other

NP_077276

Q9BUK0

Human

MGSSHHHHHH SSGLVPRGSH MGSMHQTRTG KKTVRMPSVT QRLRDPDINP CLSESDASTR CLDENNYDRE RCSTYFLRYK NCRRFWNSIV MQRRKNGVKP FMPTAAERDE ILRAVGNMPY