Recombinant humaan Chitinase 3-achtig 1/CHI3L1-eiwit

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. CHI3L1 is a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. This protein is thought to play a role in the process of inflammation and tissue remodeling. Recombinant human CHI3L1 protein was expressed with C-terminal myc-His-tag in CHO (chinese hamster ovary) cells using mammalian expression system and purified by using conventional chromatography techniques.

Chitinase 3-like 1, cartilage glycoprotein-39, CGP-39; GP-39; hCGP-39, YKL40, YKL-40, YK-40, 39 kDa synovial protein, ASRT7

CHO Cell

Human

His-Myc-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

43.3 kDa (387aa)

Chitinase3-like, Chitinase 3-like 1, GP39, ASRT7, YKL40, YYL-40, HC-gp39, HCGP-3P, ATGP1962-10 µg, ATGP1962-20 µg, ATGP1962-50 µg, ATGP1962-100 µg, ATGP1962-250 µg, ATGP1962-500 µg, ATGP1962-1 mg, ATGP1962-10, ATGP1962-20, ATGP1962-50, ATGP1962-100, ATGP1962-250, ATGP1962-500, ATGP1962-1

Kazakova MH. et al. (2009) Folia Med. 51:5-14.; ; Bonneh-Barkay D. et al. (2008) Am J Pathol. 173:130-143.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Signal Transduction

NP_001267

P36222

Human