Recombinant humaan CHMP1A eiwit

Charged multivesicular body protein 1a, also known as CHMP1A, belongs to the vacuolar sorting protein family and functions as chromatin-modifying protein. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), as well as required for the formation of these bodies. The MVBs pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. CHMP1 interacts with VPS4B and localizes to early endosomes. Two isoforms, encoded by distinct genes, exists for CHMP1. They are designated CHMP1A and CHMP1B. Recombinant human CHMP1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Charged multivesicular body protein 1a, CHMP1, PCOLN3, PRSM1, VPS46-1, VPS46A, Chromatin-modifying protein 1a, CHMP1a, Vacuolar protein sorting-associated protein 46-1, hVps46-1, Procollagen type III N-endopeptidase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.1 kDa (219aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CHMP1A, Charged multivesicular body protein 1a, CHMP1, PCOLN3, PRSM1, VPS46-1, VPS46A, ATGP1841-10 µg, ATGP1841-20 µg, ATGP1841-50 µg, ATGP1841-100 µg, ATGP1841-250 µg, ATGP1841-500 µg, ATGP1841-1 mg, ATGP1841-10, ATGP1841-20, ATGP1841-50, ATGP1841-100, ATGP1841-250, ATGP1841-500, ATGP1841-1

Stauffer D R., et al. (2001) J Cell Sci. 114:2383-2393; ; Howard T L., et al. (2001) J Cell Sci. 114: 2395-2404.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Cell Cycle

NP_002759.2

Q9HD42

Human

MDDTLFQ LKFTAKQLEK LAKKAEKDSK AEQAKVKKAL LQKNVECARV YAENAIRKKN EGVNWLRMAS RVDAVASKVQ TAVTMKGVTK NMAQVTKALD KALSTMDLQK VSSVMDRFEQ QVQNLDVHTS VMEDSMSSAT TLTTPQEQVD SLIMQIAEEN GLEVLDQLSQ LPEGASAVGE SSVRSQEDQL SRRLAALRN