Recombinant humaan CHMP1B eiwit

CHMP1B, also known as charged multivesicular body protein 1b, is probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Recombinant human CHMP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Charged multivesicular body protein 1B, CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2, C18orf2, Vps46B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.7 kDa (223aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CHMP1B, Charged multivesicular body protein 1b, C10orf2, C18-ORF2, C18orf2, CHMP1.5, hVps46-2, Vps46-2, Vps46B, ATGP1571-10 µg, ATGP1571-20 µg, ATGP1571-50 µg, ATGP1571-100 µg, ATGP1571-250 µg, ATGP1571-500 µg, ATGP1571-1 mg, ATGP1571-10, ATGP1571-20, ATGP1571-50, ATGP1571-100, ATGP1571-250, ATGP1571-500, ATGP1571-1

Martin-Serrano J., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:12414-12419; ; Bajorek M., et al. (2009) Mol. Biol. Cell. 20:1360-1373

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Cell Cycle

NP_065145.2

Q7LBR1

Human

MSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAI RQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLD VQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV GTSVASAEQD ELSQRLARLR DQV