Recombinant humaan CHMP2A eiwit

CHMP2A, also known as chromatin modifying protein 2A, belongs to the SNF7 family and it is component of the ESCRT III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. Recombinant CHMP2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Charged multivesicular body protein 2A, Chromatin-modifying protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1, BC-2, CHMP2, VPS2A, VPS2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.2 kDa (242aa) confirmed by MALDI-TOF

CHMP2A, Chromatin modifying protein 2A, BC-2, BC2, CHMP2, VPS2, VPS2A, Chromatin modifying protein 2A Charged multivesicular body protein 2a Vps2 1, hVps2 1, putative breast adenocarcinoma marker, Putative breast adenocarcinoma marker BC2, Vacuolar protein sorting associated protein 2 1., ATGP0370-10 µg, ATGP0370-20 µg, ATGP0370-50 µg, ATGP0370-100 µg, ATGP0370-250 µg, ATGP0370-500 µg, ATGP0370-1 mg, ATGP0370-10, ATGP0370-20, ATGP0370-50, ATGP0370-100, ATGP0370-250, ATGP0370-500, ATGP0370-1

Lata S., et al. (2008) Science. 321 (5894) :1354-7. ; ; Agromayor M., et al. (2006) J Biol Chem. 281 (32) :23083-91.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 30% glycerol

Metabolism

NP_055268.1

O43633

Human

MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD