Recombinant humaan CHMP2B eiwit

CHMP2B belongs to the vacuolar sorting protein family. It is a component of the ESCRT-III complex. This complex is crucial for sorting endosomal articles into multivesicular bodies (MVBs), and are also required for the formation of these bodies. CHMP2B widely expressed in brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta and leukocytes. Recombinant human CHMP2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Charged multivesicular body protein 2B, Charged multivesicular body protein 2b, CHMP2.5, Chromatin-modifying protein 2b, CHMP2b, Vacuolar protein sorting-associated protein 2-2, Vps2-2, hVps2-2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.1 kDa (233aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CHMP2B, Chromatin modifying protein 2B, DMT1, VPS2B, VPS2-2, CHMP2.5, DKFZp564O123, ATGP1115-10 µg, ATGP1115-20 µg, ATGP1115-50 µg, ATGP1115-100 µg, ATGP1115-250 µg, ATGP1115-500 µg, ATGP1115-1 mg, ATGP1115-10, ATGP1115-20, ATGP1115-50, ATGP1115-100, ATGP1115-250, ATGP1115-500, ATGP1115-1

Rizzu P., et al. (2006) Am J Med Genet B Neuropsychiatr Genet. 141B (8) :944-6.; ; Stuchell-Brereton MD., et al. (2007) Nature. 449 (7163) :740-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Neuroscience

NP_054762.2

Q9UQN3

Human

MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD EEIERQLKAL GVD