Recombinant humaan CHMP4A eiwit

CHMP4A (Chromatin-modifying protein 4a) belongs to the SNF7 family and functions as chromatin-modifying proteins. It probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Also during HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins via the association of CHMP4 and a protein recruited by HIV-1 p6, which is present in viral Gag assembly and budding. CHMP4A expressed at higher level in heart, kidney, liver and skeletal muscle. Recombinant human CHMP4A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Charged multivesicular body protein 4A, Chromatin-modifying protein 4a, CHMP4a, SNF7 homolog associated with Alix-2, SNF7-1, hSnf-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, hVps32-1, HSPC134, VPS32A

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

31.9kDa (285aa) confirmed by MALDI-TOF

CHMP4A, chromatin modifying protein 4A, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A, ATGP1376-10 µg, ATGP1376-20 µg, ATGP1376-50 µg, ATGP1376-100 µg, ATGP1376-250 µg, ATGP1376-500 µg, ATGP1376-1 mg, ATGP1376-10, ATGP1376-20, ATGP1376-50, ATGP1376-100, ATGP1376-250, ATGP1376-500, ATGP1376-1

Katoh K., et al. (2004) Arch. Biochem. Biophys. 421:159-165; ; Strack B., et al (2003) Cell 114:689-699

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200 mM NaCl, 0.1mM PMSF, 1mM EDTA

Metabolism

NP_054888

Q9BY43

Human

MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS