Recombinant humaan CHMP5 eiwit

CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs) . Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression. Recombinant human CHMP5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Charged multivesicular body protein 5, Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60, Vps60, hVps60, C9orf83, SNF7DC2, HSPC177, CGI-34

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

27 kDa (243aa) confirmed by MALDI-TOF

CHMP5, Charged multivesicular body protein 5, C9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, Vps60, ATGP1740-10 µg, ATGP1740-20 µg, ATGP1740-50 µg, ATGP1740-100 µg, ATGP1740-250 µg, ATGP1740-500 µg, ATGP1740-1 mg, ATGP1740-10, ATGP1740-20, ATGP1740-50, ATGP1740-100, ATGP1740-250, ATGP1740-500, ATGP1740-1

Howard TL, Stauffer DR, et al. (2001) . J Cell Sci. 2395-404.; ; Ward DM, Vaughn MB, et al. (2005) . J Biol Chem. 18; ;280 (11) :10548-55. Epub 2005 Jan 11.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol

Signal Transduction

NP_057494

Q9NZZ3

Human

MNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS