Recombinant humaan Chromogranine A/CHGA-eiwit

Chromogranin A is a member of the chromogranin/secretogranin (granins) family of neuroendocrine secretory proteins. Chromogranin A (CgA) represents the major soluble protein co-stored and co-released with catecholamines, within chromaffin cells secretory granules. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. It is considered a diagnostic and prognostic marker of several diseases, including a variety of tumours and cardiac heart failure. Recombinant human Chromogranin A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Cga, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin-2, EA-92, ES-43, Pancreastatin, parastatin, PHE5, PHES, SS-18, WA-8, WE-14, LF-19, Catestatin 2, SL21, AL-11, GV-19, GR-44, ER-37, GE-25, Serpinin-RRG, Serpinin, p-Glu serpinin precursor

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

51.2 kDa (460aa) (Molecular weight on SDS-PAGE will appear higher)

Chromogranin A, ATGP0323-10 µg, ATGP0323-20 µg, ATGP0323-50 µg, ATGP0323-100 µg, ATGP0323-250 µg, ATGP0323-500 µg, ATGP0323-1 mg, ATGP0323-10, ATGP0323-20, ATGP0323-50, ATGP0323-100, ATGP0323-250, ATGP0323-500, ATGP0323-1

Montero-Hadjadje M., et al. (2009) . J Biol Chem. 284 (18) :12420-31.; ; Hendy GN., et al. (1995) . Clin Invest Med. 18 (1) :47-65;

Carmon O, et al. Chromogranin A preferential interaction with Golgi phosphatidic acid induces membrane deformation and contributes to secretory granule biogenesis. (FASEB J. 2020) {https://pubmed.ncbi.nlm.nih.gov/32227388/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 0.1mM PMSF, 10% glycerol

Cancer

NP_001266.1

P10645

Human

LPVNSPMNK GDTEVMKCIV EVISDTLSKP SPMPVSQECF ETLRGDERIL SILRHQNLLK ELQDLALQGA KERAHQQKKH SGFEDELSEV LENQSSQAEL KEAVEEPSSK DVMEKREDSK EAEKSGEATD GARPQALPEP MQESKAEGNN QAPGEEEEEE EEATNTHPPA SLPSQKYPGP QAEGDSEGLS QGLVDREKGL SAEPGWQAKR EEEEEEEEEA EAGEEAVPEE EGPTVVLNPH PSLGYKEIRK GESRSEALAV DGAGKPGAEE AQDPEGKGEQ EHSQQKEEEE EMAVVPQGLF RGGKSGELEQ EEERLSKEWE DSKRWSKMDQ LAKELTAEKR LEGQEEEEDN RDSSMKLSFR ARAYGFRGPG PQLRRGWRPS SREDSLEAGL PLQVRGYPEE KKEEEGSANR RPEDQELESL SAIEAELEKV AHQLQALRRG