Recombinant humaan Chromogranine A/CHGA-eiwit

CHGA, also known as chromogranin-A isoform 1 preproprotein, is a member of neuroendocrine secretory proteins of the chromogranin / secretogranin family. It is a precursor of several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. This peptide negatively regulates the neuroendocrine function of autocrine or nearby cells (paracrine) . This protein induces and promotes the production of secretory granules containing insulin in pancreatic islet beta cells. Recombinant human CHGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Cga, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin-2, EA-92, ES-43, Pancreastatin, parastatin, PHE5, PHES, SS-18, WA-8, WE-14, LF-19, Catestatin 2, SL21, AL-11, GV-19, GR-44, ER-37, GE-25, Serpinin-RRG, Serpinin, p-Glu serpinin precursor

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

50kDa (448aa)

Vasostatin2, Vasostatin 2, Vasostatin, SP-I, SPI, SP-1, SP1, SP I, SP 1, Pituitary secretory protein I, Parathyroid secretory protein 1, Parastatin, Pancreastatin, Chromogranin-A isoform 1, Chromogranin-A, ChromograninA, Chromogranin A precursor, Chromogranin A parathyroid secretory protein 1, Chromogranin A isoform 1, Chromogranin A, CHGA, CHG A, CGA, beta Granin, Chromogranin-A precursor, Chromogranin-A parathyroid secretory protein 1, ATGP3728-10 µg, ATGP3728-20 µg, ATGP3728-50 µg, ATGP3728-100 µg, ATGP3728-250 µg, ATGP3728-500 µg, ATGP3728-1 mg, ATGP3728-010, ATGP3728-020, ATGP3728-050, ATGP3728-100, ATGP3728-250, ATGP3728-500, ATGP3728-01M

Nikoopour E., et al. (2011) J Immunol. 186:3831-3835.; ; Stadinski BD., et al. (2010) Nat Immunol. 11:225-231.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 0.1mM PMSF

Neuroscience

NP_001266.1

P10645

Human

Ala

LPVNSPM NKGDTEVMKC IVEVISDTLS KPSPMPVSQE CFETLRGDER ILSILRHQNL LKELQDLALQ GAKERAHQQK KHSGFEDELS EVLENQSSQA ELKEAVEEPS SKDVMEKRED SKEAEKSGEA TDGARPQALP EPMQESKAEG NNQAPGEEEE EEEEATNTHP PASLPSQKYP GPQAEGDSEG LSQGLVDREK GLSAEPGWQA KREEEEEEEE EAEAGEEAVP EEEGPTVVLN PHPSLGYKEI RKGESRSEAL AVDGAGKPGA EEAQDPEGKG EQEHSQQKEE EEEMAVVPQG LFRGGKSGEL EQEEERLSKE WEDSKRWSKM DQLAKELTAE KRLEGQEEEE DNRDSSMKLS FRARAYGFRG PGPQLRRGWR PSSREDSLEA GLPLQVRGYP EEKKEEEGSA NRRPEDQELE SLSAIEAELE KVAHQLQALR RG