Recombinant humaan CIAO1-eiwit

CIAO1 is essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. This protein is required for the maturation of extra mitochondrial Fe/S proteins and seems to specifically modulate the trans-activation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Recombinant human CIAO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

40 kDa (362aa) confirmed by MALDI-TOF

CIAO1, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1, WDR39, ATGP2006-10 µg, ATGP2006-20 µg, ATGP2006-50 µg, ATGP2006-100 µg, ATGP2006-250 µg, ATGP2006-500 µg, ATGP2006-1 mg, ATGP2006-10, ATGP2006-20, ATGP2006-50, ATGP2006-100, ATGP2006-250, ATGP2006-500, ATGP2006-1

Johnstone RW, Wang J, et al. (1998) . J Biol Chem. 273 (18) :10880-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004795

O76071

Human

MKDSLVL LGRVPAHPDS RCWFLAWNPA GTLLASCGGD RRIRIWGTEG DSWICKSVLS EGHQRTVRKV AWSPCGNYLA SASFDATTCI WKKNQDDFEC VTTLEGHENE VKSVAWAPSG NLLATCSRDK SVWVWEVDEE DEYECVSVLN SHTQDVKHVV WHPSQELLAS ASYDDTVKLY REEEDDWVCC ATLEGHESTV WSLAFDPSGQ RLASCSDDRT VRIWRQYLPG NEQGVACSGS DPSWKCICTL SGFHSRTIYD IAWCQLTGAL ATACGDDAIR VFQEDPNSDP QQPTFSLTAH LHQAHSQDVN CVAWNPKEPG LLASCSDDGE VAFWKYQRPE GL