Recombinant humaan CIRBP-eiwit

Cold inducible RNA-binding protein, also known as CIRBP, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. It is localizes to the nucleus and contains one RRM (RNA recognition motif) domain. Expressed ubiquitously, CIRBP is thought to play an essential role in the suppression of cellular proliferation in response to uV irradiation or extreme cold. Recombinant human CIRBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cold inducible RNA-binding protein, CIRP, A18HNRNP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.8 kDa (192aa) confirmed by MALDI-TOF

CIRBP, ATGP1033-10 µg, ATGP1033-20 µg, ATGP1033-50 µg, ATGP1033-100 µg, ATGP1033-250 µg, ATGP1033-500 µg, ATGP1033-1 mg, ATGP1033-10, ATGP1033-20, ATGP1033-50, ATGP1033-100, ATGP1033-250, ATGP1033-500, ATGP1033-1

Sheikh MS., et al. (1997) J Biol Chem. 272 (42) :26720-6; ; Nishiyama H., et al. (1997) Gene. 204:115-120.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl

Epigenomics (Transcription & Translation)

NP_001271

Q14011

Human

MASDEGKLFV GGLSFDTNEQ SLEQVFSKYG QISEVVVVKD RETQRSRGFG FVTFENIDDA KDAMMAMNGK SVDGRQIRVD QAGKSSDNRS RGYRGGSAGG RGFFRGGRGR GRGFSRGGGD RGYGGNRFES RSGGYGGSRD YYSSRSQSGG YSDRSSGGSY RDSYDSYATH NE