Recombinant humaan CISD1-eiwit

CISD1 is a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Recombinant human CISD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DGSH iron-sulfur domain-containing protein 1, Cysteine transaminase CISD1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

11.4 kDa (100aa) confirmed by MALDI-TOF

CISD1, ATGP1721-10 µg, ATGP1721-20 µg, ATGP1721-50 µg, ATGP1721-100 µg, ATGP1721-250 µg, ATGP1721-500 µg, ATGP1721-1 mg, ATGP1721-10, ATGP1721-20, ATGP1721-50, ATGP1721-100, ATGP1721-250, ATGP1721-500, ATGP1721-1

Zuris JA., et al. (2011) . Proc Natl Acad Sci u S A. 9 108 (32) :13047-52. ; ; Conlan AR., et al. (2011) . Acta Crystallogr D Biol Crystallogr. 67 (Pt 6) :516-23.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_060934

Q9NZ45

Human

KRFYVKD HRNKAMINLH IQKDNPKIVH AFDMEDLGDK AVYCRCWRSK KFPFCDGAHT KHNEETGDNV GPLIIKKKET