Recombinant humaan CITED2-eiwit

CITED2 is an important transcriptional cofactor involved in multiple organ development. It is a cAMP-responsive element-binding protein (CBP) /p300 interacting transcriptional modulator and a proposed negative regulator for hypoxia-inducible factor (HIF) -1alpha through its competitive binding with HIF-1alpha to CBP/p300. CITED2 is necessary for mouse fetal liver hematopoiesis and is required for the proper formation of the hyaloid vasculature and for lens morphogenesis. Recombinant CITED2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Cbp/p300-interacting transactivator 2, MRG1, P35SRJ, CITED2, Cbp/p300-interacting transactivator 2 Cbp/p300 interacting transactivator 2, Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2, MRG 1, MSG related protein 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

29.5 kDa (278aa) confirmed by MALDI-TOF

CITED2, Cbp/p300-interacting transactivator 2, Cbp/p300-interacting transactivator 2, MRG1, P35SRJ, CITED2, Cbp/p300-interacting transactivator 2 Cbp/p300 interacting transactivator 2, Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2, MRG 1, MSG related protein 1, ATGP0326-10 µg, ATGP0326-20 µg, ATGP0326-50 µg, ATGP0326-100 µg, ATGP0326-250 µg, ATGP0326-500 µg, ATGP0326-1 mg, ATGP0326-10, ATGP0326-20, ATGP0326-50, ATGP0326-100, ATGP0326-250, ATGP0326-500, ATGP0326-1

Bijl J., et al. (2006) Blood. 108 (1) :116-22. ; ; Chen Y., et al. (2008) Development. 135 (17) :2939-48.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 50% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_006070.2

Q99967

Human