Recombinant humaan CKMT1A-eiwit

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Recombinant human CKMT1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37C.

Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that convert 1.0 umole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37C.

45 kDa (403aa) confirmed by MALDI-TOF

CKMT1A, Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK, ATGP1590-10 µg, ATGP1590-20 µg, ATGP1590-50 µg, ATGP1590-100 µg, ATGP1590-250 µg, ATGP1590-500 µg, ATGP1590-1 mg, ATGP1590-10, ATGP1590-20, ATGP1590-50, ATGP1590-100, ATGP1590-250, ATGP1590-500, ATGP1590-1

Stachowiak O., et al. (1998) . Mol. Cell. Biochem. 184 (1-2) : 141-51.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Metabolism

NP_001015001

P12532

Human

MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH