Recombinant humaan CKMT2-eiwit

CKMT2, as known as SMTCK, belongs to the ATP:guanido phosphotransferase family. SMTCK is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. This enzyme is reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e. g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Recombinant human CKMT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Creatine kinase S-type mitochondrial, Creatine kinase S-type, mitochondrial, SMTCK

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

46.1 kDa (405aa) confirmed by MALDI-TOF

CKMT2, Creatine kinase S-type mitochondrial, Creatine kinase S-type, mitochondrial, SMTCK, ATGP1599-10 µg, ATGP1599-20 µg, ATGP1599-50 µg, ATGP1599-100 µg, ATGP1599-250 µg, ATGP1599-500 µg, ATGP1599-1 mg, ATGP1599-10, ATGP1599-20, ATGP1599-50, ATGP1599-100, ATGP1599-250, ATGP1599-500, ATGP1599-1

Payne RM, et al. (1995) Mol. Cell. Biochem. 133-134: 235-43. ; ; Qin W, Khuchua Z, et al. (1998) Mol. Cell. Biochem. 184 (1-2) : 153-67.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Metabolism

NP_001093205

P17540

Human

EVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WPDARGIWHN YDKTFLIWIN EEDHTRVISM EKGGNMKRVF ERFCRGLKEV ERLIQERGWE FMWNERLGYI LTCPSNLGTG LRAGVHVRIP KLSKDPRFSK ILENLRLQKR GTGGVDTAAV ADVYDISNID RIGRSEVELV QIVIDGVNYL VDCEKKLERG QDIKVPPPLP QFGKK