Recombinant humaan CKS1B-eiwit

CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. Recombinant human CKS1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cyclin-dependent kinases regulatory subunit 1, CDC28 protein kinase regulatory subunit 1B, CKS1, ckshs1, PNAS-16, PNAS-18

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

12.0 kDa (102aa) confirmed by MALDI-TOF

CKS1B, Cyclin-dependent kinases regulatory subunit 1, CDC28 protein kinase regulatory subunit 1B, CKS1, ckshs1, PNAS-16, PNAS-18, ATGP2511-10 µg, ATGP2511-20 µg, ATGP2511-50 µg, ATGP2511-100 µg, ATGP2511-250 µg, ATGP2511-500 µg, ATGP2511-1 mg, ATGP2511-10, ATGP2511-20, ATGP2511-50, ATGP2511-100, ATGP2511-250, ATGP2511-500, ATGP2511-1

Richardson H.E., Stueland C.S., et al. (1990) Genes Dev. 4:1332-1344

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Cancer

NP_001817

P61024

Human

MGSSHHHHHH SSGLVPRGSH MGSMSHKQIY YSDKYDDEEF EYRHVMLPKD IAKLVPKTHL MSESEWRNLG VQQSQGWVHY MIHEPEPHIL LFRRPLPKKP KK