Recombinant humaan CLIC1-eiwit

CLIC1 (Chloride intracellular channel 1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. This protein is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as an anion-selective channel. It forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring. Recombinant human CLIC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Chloride intracellular channel protein 1, G6, NCC27, Chloride intracellular channel protein 1 hRNCC, Nuclear chloride ion channel 27, p64CLCP, Chloride channel ABP, Chloride intracellular channel 1, CLIC 1, NCC 27, Nuclear chloride ion channel protein, Regulatory nuclear chloride ion channel protein, RNCC protein.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

29.0 kDa (261aa), confirmed by MALDI-TOF

CLIC1, ATGP0568-10 µg, ATGP0568-20 µg, ATGP0568-50 µg, ATGP0568-100 µg, ATGP0568-250 µg, ATGP0568-500 µg, ATGP0568-1 mg, ATGP0568-10, ATGP0568-20, ATGP0568-50, ATGP0568-100, ATGP0568-250, ATGP0568-500, ATGP0568-1

Warton K, et al. (2002) J Biol Chem. 277 (29) :26003-11.; ; Singh H, et al. (2007) FEBS J. 274 (24) :6306-16;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Signal Transduction

NP_001279.2

O00299

Human

MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP GGQLPFLLYG TEVHTDTNKI EEFLEAVLCP PRYPKLAALN PESNTAGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GVSQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVAKAL K