Recombinant humaan CLIC4-eiwit

CLIC4 (chloride intracellular channel 4), also known as H1, CLIC4L or MTCLIC, is a 253 amino acid single-pass membrane protein that localizes to both the nucleus and the cytoplasm and contains one GST C-terminal domain. CLIC4 functions as a monomer that is able to form selective ion channels in target proteins, thereby facilitating the transport of chloride and other ions. CLIC4 is thought to play a role in apoptosis and has been shown to translocate to the nucleus under stress conditions. Recombinant human CLIC4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Chloride intracellular channel protein 4, CLIC4L, DKFZp566G223, FLJ38640, H1, huH1, MTCLIC, P64h1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.9 kDa (273aa) confirmed by MALDI-TOF

CLIC4, Chloride intracellular channel protein 4, CLIC4L, DKFZp566G223, FLJ38640, H1, huH1, MTCLIC, P64h1, ATGP0961-10 µg, ATGP0961-20 µg, ATGP0961-50 µg, ATGP0961-100 µg, ATGP0961-250 µg, ATGP0961-500 µg, ATGP0961-1 mg, ATGP0961-10, ATGP0961-20, ATGP0961-50, ATGP0961-100, ATGP0961-250, ATGP0961-500, ATGP0961-1

Malik M., et al. (2010) J Biol Chem. 285 (31) :23818-28.; ; Shukla A., et al. (2009) Nat Cell Biol. 11 (6) :777-84.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Apoptosis

NP_039234

Q9Y696

Human

MGSSHHHHHH SSGLVPRGSH MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRKFLD GNEMTLADCN LLPKLHIVKV VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR LTK