Recombinant humaan CLNS1A-eiwit

CLNS1A, also as known as, Methylosome subunit pICln, belongs to the pICln (TC 1. A. 47) family. CLNS1A protein has functions in multiple regulatory pathways. This protein interaction with Sm proteins inhibits their assembly on u RNA and interferes with snRNP biogenesis. It is Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. And this protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Recombinant human CLNS1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methylosome subunit pICln, CLCI, CLNS1B, Icln

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.7 kDa (261aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CLNS1A, Methylosome subunit pICln, CLCI, CLNS1B, Icln, ATGP1799-10 µg, ATGP1799-20 µg, ATGP1799-50 µg, ATGP1799-100 µg, ATGP1799-250 µg, ATGP1799-500 µg, ATGP1799-1 mg, ATGP1799-10, ATGP1799-20, ATGP1799-50, ATGP1799-100, ATGP1799-250, ATGP1799-500, ATGP1799-1

Pu W.T., et al. (1999) Mol. Cell. Biol. 19:4113-4120; ; Friesen W.J., et al. (2001) Mol. Cell. Biol. 21:8289-8300;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_001284

P54105

Human

MGSHMSFLKS FPPPGPAEGL LRQQPDTEAV LNGKGLGTGT LYIAESRLSW LDGSGLGFSL EYPTISLHAL SRDRSDCLGE HLYVMVNAKF EEESKEPVAD EEEEDSDDDV EPITEFRFVP SDKSALEAMF TAMCECQALH PDPEDEDSDD YDGEEYDVEA HEQGQGDIPT FYTYEEGLSH LTAEGQATLE RLEGMLSQSV SSQYNMAGVR TEDSIRDYED GMEVDTTPTV AGQFEDADVD H