Recombinant humaan CLPP-eiwit

CLPP (ATP-dependent Clp protease proteolytic subunit) belongs to the peptidase family S14. CLPP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLPP, the catalytic core of the Clp proteolytic complex, is widely involved in many cellular processes via the regulation of intracellular protein quality. CLPP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Recombinant human CLPP protein was expressed in E. coli and purified by using conventional chromatography techniques.

Putative ATP-dependent Clp protease proteolytic subunit mitochondrial, Endopeptidase Clp

E.coli

Human

Non-Tagged

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.2 kDa (222aa) confirmed by MALDI-TOF

CLPP, Putative ATP-dependent Clp protease proteolytic subunit mitochondrial, Endopeptidase Clp, ATGP1073-10 µg, ATGP1073-20 µg, ATGP1073-50 µg, ATGP1073-100 µg, ATGP1073-250 µg, ATGP1073-500 µg, ATGP1073-1 mg, ATGP1073-10, ATGP1073-20, ATGP1073-50, ATGP1073-100, ATGP1073-250, ATGP1073-500, ATGP1073-1

Li XH., et al (2010) BMC Microbiol. 19:10:54.; ; Kang, S.G., et al (2002) J. Biol. Chem. 277: 21095-21102.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Metabolism

NP_006003.1

Q16740

Human

MPLIPIVVEQ TGRGERAYDI YSRLLRERIV CVMGPIDDSV ASLVIAQLLF LQSESNKKPI HMYINSPGGV VTAGLAIYDT MQYILNPICT WCVGQAASMG SLLLAAGTPG MRHSLPNSRI MIHQPSGGAR GQATDIAIQA EEIMKLKKQL YNIYAKHTKQ SLQVIESAME RDRYMSPMEA QEFGILDKVL VHPPQDGEDE PTLVQKEPVE AAPAAEPVPA ST