Recombinant humaan CLTA-eiwit

Clathrin, light chain A, also known as CLTA, belongs to the clathrin family. Clathrin is a large, soluble protein composed of heavy and light chains. It is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are captured by Clathrin-coated vesicles. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements. Recombinant human CLTA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Clathrin light chain A, Clathrin, light chain A, LCA

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.2 kDa (242aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CLTA, Clathrin light chain A, Clathrin, light chain A, LCA, ATGP1560-10 µg, ATGP1560-20 µg, ATGP1560-50 µg, ATGP1560-100 µg, ATGP1560-250 µg, ATGP1560-500 µg, ATGP1560-1 mg, ATGP1560-10, ATGP1560-20, ATGP1560-50, ATGP1560-100, ATGP1560-250, ATGP1560-500, ATGP1560-1

Kirchhausen T., et al. (1987) Proc Natl Acad Sci uSA. 84:8805-8809.; ; Liu S H., et al. (1995) Cell. 83: 257-267.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Signal Transduction

NP_001824

P09496

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAELDP FGAPAGAPGG PALGNGVAGA GEEDPAAAFL AQQESEIAGI ENDEAFAILD GGAPGPQPHG EPPGGPDAVD GVMNGEYYQE SNGPTDSYAA ISQVDRLQSE PESIRKWREE QMERLEALDA NSRKQEAEWK EKAIKELEEW YARQDEQLQK TKANNRAAEE AFVNDIDESS PGTEWERVAR LCDFNPKSSK QAKDVSRMRS VLISLKQAPL VH