Recombinant humaan CLTB-eiwit

CLTB (Clathrin light chain B) belongs to the clathrin light chain family. Clathrin is a protein that plays a major role in the formation of coated vesicles. It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they form a polyhedral lattice that surrounds the vesicle. Coat-proteins, like clathrin, are used to build small vesicles in order to safely transport molecules between cells. Clathrin-mediated endocytosis (CME) regulates many cellular physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission. Recombinant human CLTB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Clathrin light chain B, clathrin, light chain B, LCB

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.6 kDa (234aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CLTB, clathrin light chain B, clathrin, light chain B, LCB, ATGP1662-10 µg, ATGP1662-20 µg, ATGP1662-50 µg, ATGP1662-100 µg, ATGP1662-250 µg, ATGP1662-500 µg, ATGP1662-1 mg, ATGP1662-10, ATGP1662-20, ATGP1662-50, ATGP1662-100, ATGP1662-250, ATGP1662-500, ATGP1662-1

Lisa von Kleist. et al. (2011) Cell 146:471-484; ; Chen C.-Y., et al. (2005) J. Biol. Chem. 280:6109-6117

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.2mM PMSF, 100 mM NaCl

Signal Transduction

NP_001825

P09497

Human

MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR