Recombinant humaan Clusterine/CLU-eiwit

CLu, also known as clusterin, is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants. Recombinant human CLu protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Clusterin, CLI, AAG4, APOJ, KuB1, SGP2, SGP-2, SP-40, TRPM2, TRPM-2, MGC24903

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

54.1 kDa (463aa)

Clusterin, Clusterin, CLI, AAG4, APOJ, KuB1, SGP2, SGP-2, SP-40, TRPM2, TRPM-2, MGC24903, ATGP2574-10 µg, ATGP2574-20 µg, ATGP2574-50 µg, ATGP2574-100 µg, ATGP2574-250 µg, ATGP2574-500 µg, ATGP2574-1 mg, ATGP2574-10, ATGP2574-20, ATGP2574-50, ATGP2574-100, ATGP2574-250, ATGP2574-500, ATGP2574-1

Leskov K.S., et al. (2003) J. Biol. Chem. 278:11590-11600; ; Takahashi M., et al. (2004) Oncogene. 23:9289-9294;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_001822

P10909

Human

DQTV SDNELQEMSN QGSKYVNKEI QNAVNGVKQI KTLIEKTNEE RKTLLSNLEE AKKKKEDALN ETRESETKLK ELPGVCNETM MALWEECKPC LKQTCMKFYA RVCRSGSGLV GRQLEEFLNQ SSPFYFWMNG DRIDSLLEND RQQTHMLDVM QDHFSRASSI IDELFQDRFF TREPQDTYHY LPFSLPHRRP HFFFPKSRIV RSLMPFSPYE PLNFHAMFQP FLEMIHEAQQ AMDIHFHSPA FQHPPTEFIR EGDDDRTVCR EIRHNSTGCL RMKDQCDKCR EILSVDCSTN NPSQAKLRRE LDESLQVAER LTRKYNELLK SYQWKMLNTS SLLEQLNEQF NWVSRLANLT QGEDQYYLRV TTVASHTSDS DVPSGVTEVV VKLFDSDPIT VTVPVEVSRK NPKFMETVAE KALQEYRKKH REE