Recombinant humaan CMBL eiwit

Carboxymethylenebutenolidase homolog, also known as CMBL, is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15. 2. Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Carboxymethylenebutenolidase homolog

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.6 kDa (269aa) confirmed by MALDI-TOF

CMBL, Carboxymethylenebutenolidase homolog, ATGP1648-10 µg, ATGP1648-20 µg, ATGP1648-50 µg, ATGP1648-100 µg, ATGP1648-250 µg, ATGP1648-500 µg, ATGP1648-1 mg, ATGP1648-10, ATGP1648-20, ATGP1648-50, ATGP1648-100, ATGP1648-250, ATGP1648-500, ATGP1648-1

Ravandi F., et al. (2009) Cancer. 115: 5746-5751; ; Ishizuka T., et al. (2010) J Biol Chem. 285: 11892-11902.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA

Enzymes & Proteases

NP_620164.1

Q96DG6

Human

MANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM