Recombinant humaan CMPK1-eiwit

CMPK1, also known as uMP-CMP kinase and deoxycytidylate kinase, is an enzyme that catalyzes the phosphoryl transfer from ATP to uMP, CMP and dCMP. This enzymatic reaction leads to the formation of ADP and the corresponding nucleoside diphosphate, which are required for cellular nucleic acid synthesis. It also plays an important role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. Recombinant human CMPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

UMP-CMP kinase, CMK, CMPK, RP11-511I2.1, uMK, uMP-CMPK, uMPK

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.0 kDa (248aa) confirmed by MALDI-TOF

CMPK1, uMP-CMP kinase, CMK, CMPK, RP11-511I2.1, uMK, uMP-CMPK, uMPK, ATGP0982-10 µg, ATGP0982-20 µg, ATGP0982-50 µg, ATGP0982-100 µg, ATGP0982-250 µg, ATGP0982-500 µg, ATGP0982-1 mg, ATGP0982-10, ATGP0982-20, ATGP0982-50, ATGP0982-100, ATGP0982-250, ATGP0982-500, ATGP0982-1

Van Rompay A.R., et al. (2000), Pharmacol. Ther.. 87: 189-198.; ; Liou J.Y., et al. (2002) . Cancer Res. 62: 1624-1631.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100 mM NaCl

Enzymes & Proteases

NP_057392.1

P30085

Human

MLSRCRSGLL HVLGLSFLLQ TRRPILLCSP RLMKPLVVFV LGGPGAGKGT QCARIVEKYG YTHLSAGELL RDERKNPDSQ YGELIEKYIK EGKIVPVEIT ISLLKREMDQ TMAANAQKNK FLIDGFPRNQ DNLQGWNKTM DGKADVSFVL FFDCNNEICI ERCLERGKSS GRSDDNRESL EKRIQTYLQS TKPIIDLYEE MGKVKKIDAS KSVDEVFDEV VQIFDKEG