Recombinant humaan CNBP-eiwit

CNBP is a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. It functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Recombinant human CNBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21 kDa (193aa) confirmed by MALDI-TOF

CNBP, Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9, ATGP1978-10 µg, ATGP1978-20 µg, ATGP1978-50 µg, ATGP1978-100 µg, ATGP1978-250 µg, ATGP1978-500 µg, ATGP1978-1 mg, ATGP1978-10, ATGP1978-20, ATGP1978-50, ATGP1978-100, ATGP1978-250, ATGP1978-500, ATGP1978-1

McGrath CF, Buckman JS, et al. (2003) . J Virol. 77 (15) :8524-31.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001120668

P62633

Human

MGSSHHHHHH SSGLVPRGSH MGSMSSNECF KCGRSGHWAR ECPTGGGRGR GMRSRGRGFQ FVSSSLPDIC YRCGESGHLA KDCDLQEDAC YNCGRGGHIA KDCKEPKRER EQCCYNCGKP GHLARDCDHA DEQKCYSCGE FGHIQKDCTK VKCYRCGETG HVAINCSKTS EVNCYRCGES GHLARECTIE ATA