Recombinant humaan CNOT8-eiwit

CNOT8 belongs to the CAF1 family. It is a ubiquitous transcription factor required for a diverse set of processes and functions as part of the CCR-NOT complex. The CCR4-NOT complex functions as general transcription regulation complex. This complex contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8 and binds BTG2. Recombinant human CNOT8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CCR4-NOT transcription complex subunit 8, CAF1, CALIF, hCAF1, POP2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.9 kDa (315aa) confirmed by MALDI-TOF

CNOT8, CCR4-NOT transcription complex subunit 8, CAF1, CALIF, hCAF1, POP2, ATGP2161-10 µg, ATGP2161-20 µg, ATGP2161-50 µg, ATGP2161-100 µg, ATGP2161-250 µg, ATGP2161-500 µg, ATGP2161-1 mg, ATGP2161-10, ATGP2161-20, ATGP2161-50, ATGP2161-100, ATGP2161-250, ATGP2161-500, ATGP2161-1

Morel A.-P., et al. (2003) J. Cell Sci. 116:2929-2936; ; Albert T.K., et al. (2000) Nucleic Acids Res. 28:809-817

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004770

Q9UFF9

Human

MPAALVE NSQVICEVWA SNLEEEMRKI REIVLSYSYI AMDTEFPGVV VRPIGEFRSS IDYQYQLLRC NVDLLKIIQL GLTFTNEKGE YPSGINTWQF NFKFNLTEDM YSQDSIDLLA NSGLQFQKHE EEGIDTLHFA ELLMTSGVVL CDNVKWLSFH SGYDFGYMVK LLTDSRLPEE EHEFFHILNL FFPSIYDVKY LMKSCKNLKG GLQEVADQLD LQRIGRQHQA GSDSLLTGMA FFRMKELFFE DSIDDAKYCG RLYGLGTGVA QKQNEDVDSA QEKMSILAII NNMQQ