Recombinant humaan CNTF-eiwit

CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM) . It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E. coli and purified by using conventional chromatography techniques.

Human CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.

Ciliary neurotrophic factor

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.

22.9 kDa (200aa) confirmed by MALDI-TOF

CNTF, ATGP3316-10 µg, ATGP3316-20 µg, ATGP3316-50 µg, ATGP3316-100 µg, ATGP3316-250 µg, ATGP3316-500 µg, ATGP3316-1 mg, ATGP3316-10, ATGP3316-20, ATGP3316-50, ATGP3316-100, ATGP3316-250, ATGP3316-500, ATGP3316-1

Sendther M., et al. (1994) J Neurobiol. 25 (11) :1436-53.; ; Robert F., et al. (2007) J Biol Chem. 282 (46) :33421-33434.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Hormones & Cytokines

NP_000605

P26441

Human

MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM