Recombinant humaan CNTFR alfa-eiwit

CNTFR, also known as ciliary neurotrophic factor receptor subunit alpha preproprotein, is expressed in glial cells within the central and peripheral nervous systems. It stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. This protein has a structure unrelated to the receptors utilized by the nerve growth factor family of neurotrophic molecules, but instead is most homologous to the receptor for a cytokine, interleukin-6. Also, it possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage. It activates downstream signaling molecules such as STAT-3 in areas of the hypothalamus which regulate food intake. Recombinant human CNTFR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Ciliary neurotrophic factor receptor subunit alpha, CNTFR, CNTFR-alpha, ciliary neurotrophic factor receptor

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

36.9 kDa (329aa)

CNTF R a, Ciliary neurotrophic factor receptor subunit alpha, CNTFR, CNTFR-alpha, ciliary neurotrophic factor receptor, ATGP3830-10 µg, ATGP3830-20 µg, ATGP3830-50 µg, ATGP3830-100 µg, ATGP3830-250 µg, ATGP3830-500 µg, ATGP3830-1 mg, ATGP3830-10, ATGP3830-20, ATGP3830-50, ATGP3830-100, ATGP3830-250, ATGP3830-500, ATGP3830-1

Davis S., et al. (1991) Science. 253:59-63.; ; Sleeman MW., et al. (2000) Pharm Acta Helv. 74:265-272

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_001833

P26992

Human

Ala

ADPQRHSPQE APHVQYERLG SDVTLPCGTA NWDAAVTWRV NGTDLAPDLL NGSQLVLHGL ELGHSGLYAC FHRDSWHLRH QVLLHVGLPP REPVLSCRSN TYPKGFYCSW HLPTPTYIPN TFNVTVLHGS KIMVCEKDPA LKNRCHIRYM HLFSTIKYKV SISVSNALGH NATAITFDEF TIVKPDPPEN VVARPVPSNP RRLEVTWQTP STWPDPESFP LKFFLRYRPL ILDQWQHVEL SDGTAHTITD AYAGKEYIIQ VAAKDNEIGT WSDWSVAAHA TPWTEEPRHL TTEAQAAETT TSTTSSLAPP PTTKICDPGE LGSHHHHHH