Recombinant humaan COA4-eiwit

Cytochrome c oxidase assembly factor 4 homolog, mitochondrial, also known as COA4, is the last enzyme of the mitochondrial respiratory chain, is a very complicated process and requires the assistance of a large number of accessory factors. It is essential for oxidative phosphorylation and comprises multiple complexes, including cytochrome c oxidase, assembled in macromolecular supercomplexes. Defects in COX4 assembly alter cellular respiration and produce severe human encephalomyopathies. Recombinant human COA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytochrome c oxidase assembly factor 4 homolog mitochondrial, Cytochrome c oxidase assembly factor 4 homolog, mitochondrial, CHCHD8, CMC3, E2IG2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.5 kDa (110aa) confirmed by MALDI-TOF

COA4, Cytochrome c oxidase assembly factor 4 homolog mitochondrial, Cytochrome c oxidase assembly factor 4 homolog, mitochondrial, CHCHD8, CMC3, E2IG2, ATGP2716-10 µg, ATGP2716-20 µg, ATGP2716-50 µg, ATGP2716-100 µg, ATGP2716-250 µg, ATGP2716-500 µg, ATGP2716-1 mg, ATGP2716-10, ATGP2716-20, ATGP2716-50, ATGP2716-100, ATGP2716-250, ATGP2716-500, ATGP2716-1

Diaz F., et al. (2012) Hum Mol Genet. 1 (23) : 5066-77.; ; Huigsloot M., et al. (2011) Am J Hum Genet. 8 (4) : 488-93.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Other

NP_057649

Q9NYJ1

Human

MSTSVPQ GHTWTQRVKK DDEEEDPLDQ LISRSGCAAS HFAVQECMAQ HQDWRQCQPQ VQAFKDCMSE QQARRQEELQ RRQEQAGAHH