Recombinant humaan Coactosine-achtig eiwit 1/COTL1-eiwit

COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. COTL1 Binds to F-actin in a calcium-independent manner and has no direct effect on actin depolymerization. Recombinant human COTL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Coactosin like F-actin binding protein 1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, CLP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.1 kDa (162aa) confirmed by MALDI-TOF

CLP, Coactosin like F-actin binding protein 1, Coactosin like Protein1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, Coactosin-like protein, Coactosin-like Protein 1, Coactosin-like Protein1, COTL 1, COTL1, COTL-1, FLJ43657, MGC19733, ATGP0858-10 µg, ATGP0858-20 µg, ATGP0858-50 µg, ATGP0858-100 µg, ATGP0858-250 µg, ATGP0858-500 µg, ATGP0858-1 mg, ATGP0858-010, ATGP0858-020, ATGP0858-050, ATGP0858-100, ATGP0858-250, ATGP0858-500, ATGP0858-01M

Rakonjac M., et al. (2006) Proc Natl Acad Sci u S A. 103 (35) :13150-5. ; ; Provost P., et al. (2001) Biochem J. 359 (2) :255-63.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Cytoskeleton

NP_066972.1

Q14019

Human

MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIKSELK KAGGANYDAQ TE