Recombinant humaan cofiline 2/CFL2-eiwit

CFL2 protein belongs to the actin-binding proteins ADF family that contains 1 ADF-H domain. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) . Recombinant human CFL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CFL2, Cofilin 2, NEM7

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

20.9 kDa (186a) confirmed by MALDI-TOF

CFL2, CFL 2, CFL-2, Cofilin2, Cofilin 2, Cofilin-2, NEM7, ATGP1183-10 µg, ATGP1183-20 µg, ATGP1183-50 µg, ATGP1183-100 µg, ATGP1183-250 µg, ATGP1183-500 µg, ATGP1183-1 mg, ATGP1183-010, ATGP1183-020, ATGP1183-050, ATGP1183-100, ATGP1183-250, ATGP1183-500, ATGP1183-01M

Agrawal P.B., et al. (2007) Am. J. Hum. Genet. 80:162-167; ; Gillett GT, et al. (1996) . Ann. Hum. Genet. 60 (Pt 3) : 201-11

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 50 mM Nacl

Cytoskeleton

NP_068733

Q9Y281

Human

MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL