Recombinant humaan COPE-eiwit

COPE is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant human COPE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Coatomer subunit epsilon, Coatomer subunit epsilon, Coatomer protein complex, subunit epsilon, epsilon-COP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.9 kDa (331aa) confirmed by MALDI-TOF

COPE, Coatomer subunit epsilon, Coatomer subunit epsilon, Coatomer protein complex, subunit epsilon, epsilon-COP, ATGP2872-10 µg, ATGP2872-20 µg, ATGP2872-50 µg, ATGP2872-100 µg, ATGP2872-250 µg, ATGP2872-500 µg, ATGP2872-1 mg, ATGP2872-10, ATGP2872-20, ATGP2872-50, ATGP2872-100, ATGP2872-250, ATGP2872-500, ATGP2872-1

Shima D.T., et al. (1999) Curr. Biol. 9:821-824

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_009194

O14579

Human

MGSSHHHHHH SSGLVPRGSH MGSMAPPAPG PASGGSGEVD ELFDVKNAFY IGSYQQCINE AQRVKLSSPE RDVERDVFLY RAYLAQRKFG VVLDEIKPSS APELQAVRMF ADYLAHESRR DSIVAELDRE MSRSVDVTNT TFLLMAASIY LHDQNPDAAL RALHQGDSLE CTAMTVQILL KLDRLDLARK ELKRMQDLDE DATLTQLATA WVSLATGGEK LQDAYYIFQE MADKCSPTLL LLNGQAACHM AQGRWEAAEG LLQEALDKDS GYPETLVNLI VLSQHLGKPP EVTNRYLSQL KDAHRSHPFI KEYQAKENDF DRLVLQYAPS A