Recombinant humaan COPS7A-eiwit

COPS7A is a component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JuN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JuN promotes and protects degradation by the ubl system, respectively. Recombinant human COPS7A protein, fused to His-tag at N-terminus, was expressed in E. coli.

COP9 signalosome complex subunit 7a, COP9 signalosome complex subunit 7a, CSN7A

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

32.7 kDa (298aa)

COPS7A, COP9 signalosome complex subunit 7a, COP9 signalosome complex subunit 7a, CSN7A, ATGP2652-10 µg, ATGP2652-20 µg, ATGP2652-50 µg, ATGP2652-100 µg, ATGP2652-250 µg, ATGP2652-500 µg, ATGP2652-1 mg, ATGP2652-10, ATGP2652-20, ATGP2652-50, ATGP2652-100, ATGP2652-250, ATGP2652-500, ATGP2652-1

Wolf DA, Zhou C, et al. (2003) . Nat Cell Biol. 5 (12) :1029-33.; ; Wei N, Tsuge T, et al. (1998) . Curr Biol. 8 (16) :919-22.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Ubiquitination

NP_001157566

Q9UBW8

Human

MGSSHHHHHH SSGLVPRGSH MGSMSAEVKV TGQNQEQFLL LAKSAKGAAL ATLIHQVLEA PGVYVFGELL DMPNVRELAE SDFASTFRLL TVFAYGTYAD YLAEARNLPP LTEAQKNKLR HLSVVTLAAK VKCIPYAVLL EALALRNVRQ LEDLVIEAVY ADVLRGSLDQ RNQRLEVDYS IGRDIQRQDL SAIARTLQEW CVGCEVVLSG IEEQVSRANQ HKEQQLGLKQ QIESEVANLK KTIKVTTAAA AAATSQDPEQ HLTELREPAP GTNQRQPSKK ASKGKGLRGS AKIWSKSN