Recombinant humaan COQ9-eiwit

COQ9 also known as ubiquinone biosynthesis protein COQ9. This locus represents a mitochondrial ubiquinone biosynthesis gene. This protein is likely necessary for biosynthesis of coenzyme Q10, as mutations at this locus have been associated with autosomal-recessive neonatal-onset primary coenzyme Q10 deficiency. Recombinant human COQ9, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquinone biosynthesis protein COQ9 mitochondrial, ubiquinone biosynthesis protein COQ9, mitochondrial, C16orf49, COQ10D5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.3 kDa (297aa) confirmed by MALDI-TOF

COQ9, ubiquinone biosynthesis protein COQ9 mitochondrial, ubiquinone biosynthesis protein COQ9, mitochondrial, C16orf49, COQ10D5, ATGP3042-10 µg, ATGP3042-20 µg, ATGP3042-50 µg, ATGP3042-100 µg, ATGP3042-250 µg, ATGP3042-500 µg, ATGP3042-1 mg, ATGP3042-10, ATGP3042-20, ATGP3042-50, ATGP3042-100, ATGP3042-250, ATGP3042-500, ATGP3042-1

Duncan AJ., et al. (2009) Am. J. Hum. Genet. 84 (5), 558-566

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Ubiquitination

NP_064708

O75208

Human

MGSSHHHHHH SSGLVPRGSH MGSRSSDEQK QQPPNSFSQQ HSETQGAEKP DPESSHSPPR YTDQGGEEEE DYESEEQLQH RILTAALEFV PAHGWTAEAI AEGAQSLGLS SAAASMFGKD GSELILHFVT QCNTRLTRVL EEEQKLVQLG QAEKRKTDQF LRDAVETRLR MLIPYIEHWP RALSILMLPH NIPSSLSLLT SMVDDMWHYA GDQSTDFNWY TRRAMLAAIY NTTELVMMQD SSPDFEDTWR FLENRVNDAM NMGHTAKQVK STGEALVQGL MGAAVTLKNL TGLNQRR