Recombinant humaan COX5A-eiwit

COX5A, also known as COX, COX-VA and VA, belongs to the cytochrome c oxidase subunit 5A family. Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. COX5A is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Recombinant human COX5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

14.9 kDa (132aa) confirmed by MALDI-TOF

COX5A, cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA, ATGP1867-10 µg, ATGP1867-20 µg, ATGP1867-50 µg, ATGP1867-100 µg, ATGP1867-250 µg, ATGP1867-500 µg, ATGP1867-1 mg, ATGP1867-10, ATGP1867-20, ATGP1867-50, ATGP1867-100, ATGP1867-250, ATGP1867-500, ATGP1867-1

Lenka N, et al. (1998) . Prog. Nucleic Acid Res. Mol. Biol. 61: 309-44.; ; Schmidt, T.R., et al. (2002) Gene 286: 13-19.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 2mM DTT

Metabolism

NP_004246

P20674

Human

SHGSQET DEEFDARWVT YFNKPDIDAW ELRKGINTLV TYDMVPEPKI IDAALRACRR LNDFASTVRI LEVVKDKAGP HKEIYPYVIQ ELRPTLNELG ISTPEELGLD KV