Recombinant humaan COX5B-eiwit

Cytochrome c oxidase subunit 5B, also known as COX5B, is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5A (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5B (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme. Recombinant human COX5B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cytochrome c oxidase subunit 5B mitochondrial precursor, Cytochrome c oxidase subunit 5B, mitochondrial precursor, COXVB

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

13 kDa (121aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

COX5B, Cytochrome c oxidase subunit 5B mitochondrial precursor, Cytochrome c oxidase subunit 5B, mitochondrial precursor, COXVB, ATGP2156-10 µg, ATGP2156-20 µg, ATGP2156-50 µg, ATGP2156-100 µg, ATGP2156-250 µg, ATGP2156-500 µg, ATGP2156-1 mg, ATGP2156-10, ATGP2156-20, ATGP2156-50, ATGP2156-100, ATGP2156-250, ATGP2156-500, ATGP2156-1

Bueke P V., et al. (1998) J Exp Biol. 201:1163-1175.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_001853

P10606

Human

MGSSHHHHHH SSGLVPRGSH MGSASGGGVP TDEEQATGLE REIMLAAKKG LDPYNVLAPK GASGTREDPN LVPSISNKRI VGCICEEDNT SVVWFWLHKG EAQRCPRCGA HYKLVPQQLA H