Recombinant humaan CPOX-eiwit

Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria. Recombinant human CPOX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

41.6 kDa (367aa) confirmed by MALDI-TOF

CPOX, Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP, ATGP2042-10 µg, ATGP2042-20 µg, ATGP2042-50 µg, ATGP2042-100 µg, ATGP2042-250 µg, ATGP2042-500 µg, ATGP2042-1 mg, ATGP2042-10, ATGP2042-20, ATGP2042-50, ATGP2042-100, ATGP2042-250, ATGP2042-500, ATGP2042-1

Martasek P., et al. (1994) Hum Mol Genet. 3:477-480; ; Lamoril J., et al. (1995) Hum Mol Genet. 4:275-278.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_000088

P36551

Human

MGSSHHHHHH SSGLVPRGSH MGSTSLGRPE EEEDELAHRC SSFMAPPVTD LGELRRRPGD MKTKMELLIL ETQAQVCQAL AQVDGGANFS VDRWERKEGG GGISCVLQDG CVFEKAGVSI SVVHGNLSEE AAKQMRSRGK VLKTKDGKLP FCAMGVSSVI HPKNPHAPTI HFNYRYFEVE EADGNKQWWF GGGCDLTPTY LNQEDAVHFH RTLKEACDQH GPDLYPKFKK WCDDYFFIAH RGERRGIGGI FFDDLDSPSK EEVFRFVQSC ARAVVPSYIP LVKKHCDDSF TPQEKLWQQL RRGRYVEFNL LYDRGTKFGL FTPGSRIESI LMSLPLTARW EYMHSPSENS KEAEILEVLR HPRDWVR