Recombinant humaan creatininekinase B/CKB-eiwit

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells. Recombinant human CKB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography technique

Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

44.8 kDa (401aa) confirmed by MALDI-TOF

Creatine Kinase BB, CKB, Creatine kinase B-type, B-CK, CKBB, Creatine kinase B-type, BCK, ATGP0626-10 µg, ATGP0626-20 µg, ATGP0626-50 µg, ATGP0626-100 µg, ATGP0626-250 µg, ATGP0626-500 µg, ATGP0626-1 mg, ATGP0626-10, ATGP0626-20, ATGP0626-50, ATGP0626-100, ATGP0626-250, ATGP0626-500, ATGP0626-1

Chang EJ., et al. (2008) Nat Med. 14 (9) :966-72. ; ; Debrincat MA., et al. (2007) J Biol Chem. 282 (7) :4728-37.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_001814

P12277

Human

MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K