Recombinant humaan creatininekinase B/CKB-eiwit

CKB, also known as Creatine Kinase B-type, is encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Recombinant human CKB, was expressed in E. coli and purified by using conventional chromatography techniques.

Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.6 kDa (381aa) confirmed by MALDI-TOF

Creatine Kinase BB, Creatine Kinase B-type, B-CK, BCK, CKBB, HEL-211, HEL-S-29, B CK, BB-CK, Brain creatine kinase, Ckb, Creatine kinase B, Creatine kinase B chain, Creatine kinase B type, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine kinase brain, Creatine phosphokinase BB, KCRB_HUMAN, ATGP3384-10 µg, ATGP3384-20 µg, ATGP3384-50 µg, ATGP3384-100 µg, ATGP3384-250 µg, ATGP3384-500 µg, ATGP3384-1 mg, ATGP3384-10, ATGP3384-20, ATGP3384-50, ATGP3384-100, ATGP3384-250, ATGP3384-500, ATGP3384-1

Steen C., et al. (2010) PLoS One. 5 (5) :e10811.; ; Hara H., et al. (2009) J Virol. 83 (10) 5137-47.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Metabolism

NP_001814

P12277

Human

MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K