Recombinant humaan CREBZF-eiwit

CREBZF strongly activates transcription when bound to HCFC1. It suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. This protein suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. CREBZF is able to down-regulate expression of some cellular genes in CREBZF-expressing cells. Recombinant human CREBZF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CREB/ATF bZIP transcription factor, CREB/ATF bZIP transcription factor, SMILE, ZF

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.5 kDa (377aa) confirmed by MALDI-TOF

CREBZF, CREB/ATF bZIP transcription factor, CREB/ATF bZIP transcription factor, SMILE, ZF, ATGP2812-10 µg, ATGP2812-20 µg, ATGP2812-50 µg, ATGP2812-100 µg, ATGP2812-250 µg, ATGP2812-500 µg, ATGP2812-1 mg, ATGP2812-10, ATGP2812-20, ATGP2812-50, ATGP2812-100, ATGP2812-250, ATGP2812-500, ATGP2812-1

Lu R., Misra V.. (2002) Nucleic Acids Res. 28:2446-2454; ; Misra V., Rapin N, et al. (2005) J. Biol. Chem. 280:15257-15266.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_001034707

Q9NS37

Human

MGSSHHHHHH SSGLVPRGSH MGSMRHSLTK LLAASGSNSP TRSESPEPAA TCSLPSDLTR AAAGEEETAA AGSPGRKQQF GDEGELEAGR GSRGGVAVRA PSPEEMEEEA IASLPGEETE DMDFLSGLEL ADLLDPRQPD WHLDPGLSSP GPLSSSGGGS DSGGLWRGDD DDEAAAAEMQ RFSDLLQRLL NGIGGCSSSS DSGSAEKRRR KSPGGGGGGG SGNDNNQAAT KSPRKAAAAA ARLNRLKKKE YVMGLESRVR GLAAENQELR AENRELGKRV QALQEESRYL RAVLANETGL ARLLSRLSGV GLRLTTSLFR DSPAGDHDYA LPVGKQKQDL LEEDDSAGGV CLHVDKDKVS VEFCSACARK ASSSLKM