Recombinant humaan Crk-eiwit

CRK is a member of signaling adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an SH2 followed by two SH3 domains (src-homology domains) . The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Recombinant human CRK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Adapter molecule crk, Proto-oncogene c-Crk, p38, CRKII

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.0 kDa (224aa) confirmed by MALDI-TOF

Crk, Adapter molecule crk, Proto-oncogene c-Crk, p38, CRKII, ATGP0766-10 µg, ATGP0766-20 µg, ATGP0766-50 µg, ATGP0766-100 µg, ATGP0766-250 µg, ATGP0766-500 µg, ATGP0766-1 mg, ATGP0766-10, ATGP0766-20, ATGP0766-50, ATGP0766-100, ATGP0766-250, ATGP0766-500, ATGP0766-1

Muir TW., et al (2006) Biochemistry. 45 (29) :8874-84.; ; Inagaki F., et al. (2007) Nat Struct Mol Biol. 14 (6) :503-10.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Cancer

NP_005197

P46108

Human

MAGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL IGGR