Recombinant humaan CRKL-eiwit

CRKL is a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JuN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substitutete of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and has oncogenic potential. Recombinant human CRKL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CRK like proto-oncogene, Adaptor protein, V-crk avian sarcoma virus CT10 oncogene homolog-like

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.9 kDa (323aa) confirmed by MALDI-TOF

CrkL, Crk-like protein, ATGP0857-10 µg, ATGP0857-20 µg, ATGP0857-50 µg, ATGP0857-100 µg, ATGP0857-250 µg, ATGP0857-500 µg, ATGP0857-1 mg, ATGP0857-10, ATGP0857-20, ATGP0857-50, ATGP0857-100, ATGP0857-250, ATGP0857-500, ATGP0857-1

Arai A., et al. (2001) J Biol Chem. 276 (35) :33282-90. ; ; Senechal K., et al. (1996) J Biol Chem. 271 (38) :23255-61.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Cancer

NP_005198.1

P46109

Human

MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE