Recombinant humaan Crystalline gamma C/CRYGC-eiwit

Gamma-crystallin C, also known as CRYGC, belongs to the beta/gamma-crystallin family. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. Defects in CRYGC are a cause of cataract autosomal dominant (ADC) and cataract Coppock-like (CCL) . Recombinant human CRYGC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Gamma-crystallin C, CCL, CRYG3, Gamma-C-crystallin, Gamma-crystallin 2-1, Gamma-crystallin 3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.4 kDa (198aa) confirmed by MALDI-TOF

CRYGC, Gamma-crystallin C, CCL, CRYG3, ATGP1529-10 µg, ATGP1529-20 µg, ATGP1529-50 µg, ATGP1529-100 µg, ATGP1529-250 µg, ATGP1529-500 µg, ATGP1529-1 mg, ATGP1529-10, ATGP1529-20, ATGP1529-50, ATGP1529-100, ATGP1529-250, ATGP1529-500, ATGP1529-1

Slingsby C, et al. (2000) Eye (London, England) 13 (Pt 3b) : 395-402. ; ; Ren Z., et al. (2000) Hum. Genet. 106:531-537

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 200 mM NaCl

Neuroscience

NP_066269

P07315

Human

MGKITF YEDRAFQGRS YETTTDCPNL QPYFSRCNSI RVESGCWMLY ERPNYQGQQY LLRRGEYPDY QQWMGLSDSI RSCCLIPQTV SHRLRLYERE DHKGLMMELS EDCPSIQDRF HLSEIRSLHV LEGCWVLYEL PNYRGRQYLL RPQEYRRCQD WGAMDAKAGS LRRVVDLY