Recombinant humaan Crystalline gamma N/CRYGN-eiwit

Crystallin, gamma N, also known as CRYGN, belongs to the Crystallins family. Crystallins are divided into alpha, beta and gamma families, and the beta and gamma-crystallins also comprise a superfamily. Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. The CRYGN is differentially regulated after early development, and are involved in cataract formation as a result of either age-related protein degradation or genetic mutation. Recombinant human CRYGN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Gamma-crystallin N, Gamma-N-crystallin

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.1 kDa (206aa)

CRYGN, Crystallin gamma N, Crystallin, gamma N, gamma-crystallin N, ATGP1627-10 µg, ATGP1627-20 µg, ATGP1627-50 µg, ATGP1627-100 µg, ATGP1627-250 µg, ATGP1627-500 µg, ATGP1627-1 mg, ATGP1627-10, ATGP1627-20, ATGP1627-50, ATGP1627-100, ATGP1627-250, ATGP1627-500, ATGP1627-1

Srivastava O P., et al. (1998) Curr Eye Res. 17:1074-1081.; ; Srivastava O P., et al. (1998) Biophys Res Commun. 253:288-294.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Heat Shock Proteins

NP_653328

Q8WXF5

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAQRSG KITLYEGKHF TGQKLEVFGD CDNFQDRGFM NRVNSIHVES GAWVCFNHPD FRGQQFILEH GDYPDFFRWN SHSDHMGSCR PVGMHGEHFR LEIFEGCNFT GQCLEFLEDS PFLQSRGWVK NCVNTIKVYG DGAAWSPRSF GAEDFQLSSS LQSDQGPEEA TTKPATTQPP FLTANL