Recombinant humaan Crystalline gamma S/CRYGS-eiwit

Crystallins are water soluble structural proteins found in the vertebrate eye. Mammalian crystallins are classified in three forms, designated alplha, beta and gamma. Crystallins, as the principal components of the lens, function to increase the refractive index of the eye during accommodation by forming high-molecular weight aggregates which maintain transparency. CRYGS, also known as beta-crystallin S, is exists as a monomer which does not aggregate. This gene encodes the most significant gamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. Recombinant human CRYGS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Beta-crystallin S, CRYG8, Gamma-crystallin S, Gamma-S-crystallin

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

23.6 kDa (202aa) confirmed by MALDI-TOF

CRYGS, Beta-crystallin S, CRYG8, ATGP1205-10 µg, ATGP1205-20 µg, ATGP1205-50 µg, ATGP1205-100 µg, ATGP1205-250 µg, ATGP1205-500 µg, ATGP1205-1 mg, ATGP1205-10, ATGP1205-20, ATGP1205-50, ATGP1205-100, ATGP1205-250, ATGP1205-500, ATGP1205-1

Sun H., et al. (2005) J Med Genet. 42 (9) :706-10.; ; Jester JV., et al. (2008) Semin Cell Dev Biol. 19 (2) :82-93.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Neuroscience

NP_060011

P22914

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSKTGT KITFYEDKNF QGRRYDCDCD CADFHTYLSR CNSIKVEGGT WAVYERPNFA GYMYILPQGE YPEYQRWMGL NDRLSSCRAV HLPSGGQYKI QIFEKGDFSG QMYETTEDCP SIMEQFHMRE IHSCKVLEGV WIFYELPNYR GRQYLLDKKE YRKPIDWGAA SPAVQSFRRI VE