Recombinant humaan Crystalline zeta/CRYZ-eiwit

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. unlike other mammalian species, in humans, lens expression is low. Recombinant human CRYZ protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Quinone oxidoreductase, NADPH:quinone reductase, Zeta-crystallin

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.6 kDa (352aa) confirmed by MALDI-TOF

CRYZ, Quinone oxidoreductase isoform a, Crystallin, zeta (quinone reductase), ATGP2750-10 µg, ATGP2750-20 µg, ATGP2750-50 µg, ATGP2750-100 µg, ATGP2750-250 µg, ATGP2750-500 µg, ATGP2750-1 mg, ATGP2750-10, ATGP2750-20, ATGP2750-50, ATGP2750-100, ATGP2750-250, ATGP2750-500, ATGP2750-1

Porte S., Valencia E., et al. (2009) J. Biol. Chem. 284:17194-17205

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Neuroscience

NP_001123514

Q08257

Human

MATGQKL MRAVRVFEFG GPEVLKLRSD IAVPIPKDHQ VLIKVHACGV NPVETYIRSG TYSRKPLLPY TPGSDVAGVI EAVGDNASAF KKGDRVFTSS TISGGYAEYA LAADHTVYKL PEKLDFKQGA AIGIPYFTAY RALIHSACVK AGESVLVHGA SGGVGLAACQ IARAYGLKIL GTAGTEEGQK IVLQNGAHEV FNHREVNYID KIKKYVGEKG IDIIIEMLAN VNLSKDLSLL SHGGRVIVVG SRGTIEINPR DTMAKESSII GVTLFSSTKE EFQQYAAALQ AGMEIGWLKP VIGSQYPLEK VAEAHENIIH GSGATGKMIL LL