Recombinant humaan CTDSP1-eiwit

Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recombinant human CTDSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1, Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, NLIIF, SCP1, NIF3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.2 kDa (280aa) confirmed by MALDI-TOF

CTDSP1, Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1, Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, NLIIF, SCP1, NIF3, ATGP1013-10 µg, ATGP1013-20 µg, ATGP1013-50 µg, ATGP1013-100 µg, ATGP1013-250 µg, ATGP1013-500 µg, ATGP1013-1 mg, ATGP1013-10, ATGP1013-20, ATGP1013-50, ATGP1013-100, ATGP1013-250, ATGP1013-500, ATGP1013-1

Maiello B., et al. (2001) Front Biosci. 6: 1358-1368.; ; Palancade B., et al. (2004) J Mol Biol. 335:415-424.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Signal Transduction

NP_872580

Q9GZU7

Human

MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS