Recombinant humaan CTDSPL-eiwit

CTDSPL, also known as CTD small phosphatase-like protein, preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Recombinant human CTDSPL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

CTD small phosphatase-like protein, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase like protein

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.9 kDa (209aa) confirmed by MALDI-TOF

CTDSPL, CTD small phosphatase-like protein, HYA22, PSR1, RBSP3, SCP3, CTD small phosphatase like protein, ATGP1408-10 µg, ATGP1408-20 µg, ATGP1408-50 µg, ATGP1408-100 µg, ATGP1408-250 µg, ATGP1408-500 µg, ATGP1408-1 mg, ATGP1408-10, ATGP1408-20, ATGP1408-50, ATGP1408-100, ATGP1408-250, ATGP1408-500, ATGP1408-1

Choudhary C., et al. (2009) Science. 325:834-840; ; Daub H., et al. (2008) Mol. Cell. 31:438-448

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_005799

O15194

Human

MGSSHHHHHH SSGLVPRGSH MGSHMKYLLP EVTVLDYGKK CVVIDLDETL VHSSFKPISN ADFIVPVEID GTIHQVYVLK RPHVDEFLQR MGQLFECVLF TASLAKYADP VADLLDRWGV FRARLFRESC VFHRGNYVKD LSRLGRELSK VIIVDNSPAS YIFHPENAVP VQSWFDDMTD TELLDLIPFF EGLSREDDVY SMLHRLCNR